Preserving Plants - By: The Royal Botanic Gardens & Domain Trust

http://www.rbgsyd.nsw.gov.au/plant_info/identifying_plants/processing_plant_specimens/Preserving_plant_specimens

Pressing and drying Techniques for pressing and drying specimens have been established for many years. There are minor variations in recommended methods, but they are essentially the same worldwide. The best specimens are plants that are pressed as soon as possible after collection, before wilting and shrivelling. Most plants may be kept in sealed containers such as plastic bags for up to a day if it is inconvenient to press immediately. However, some plants show such rapid wilting, particularly of the flowers, that such delays are best avoided. Flowers with a lot of nectar may go mouldy very quickly if excess nectar is not shaken off before pressing. Specimens are pressed flat and dried between sheets of absorbent blotters or semi-absorbent paper such as newspaper. Papers with a glossy surface should be avoided because they are not absorbent enough to aid drying. The plant should be carefully laid out between the drying sheets, as their form at this stage largely determines their ultimate appearance. The flowers should be spread out with the petals carefully arranged, wilted leaves should be straightened and unnecessary shoots of excessively twiggy shrubs may be cut away. Large flowers (e.g. Nymphaea) or inflorescences (e.g. Telopea) are best cut in half lengthways before pressing. Large and/or succulent fruit is often best preserved by cutting both longitudinal and transectional (from different fruit) sections from them and drying these. Care is necessary to ensure that the maximum amount of useful information s preserved. Sheets of thick, preferably smooth-sided, centre-corrugated cardboard (such as used in cardboard carton sides), placed between the drying folders will assist air circulation through the press. These are particularly necessary when using a forced circulation of warm air. If such cardboard is not available, additional sheets of newspaper or wooden board (e.g. plywood) may be used to absorb moisture from succulent specimens. When plants are uneven in thickness, e.g. where flowers are borne on thick twigs or arise from a thick bulbous base, sheets of spongy plastic foam (polyurethane or similar) about 1 cm thick, placed between the newspaper folders, help to distribute pressure evenly across the specimen. If foam sheets are not available, several thicknesses of folded newspaper may be used. Care must be taken to ensure 'damp spots' do not develop in the press. When using foam sheets it is advisable to circulate warm air around the press or change the drying papers more frequently. Specimens are best pressed with moderate pressure, preferably in an arrangement that will permit as free a circulation of air as possible. This can be achieved by strapping the pile together in a press, i.e. between frames made, for example, from sheets of heavy (non-bending) cardboard, hardboard, plywood, pegboard or, best of all, a lattice of wood or weldmesh (see picture). Supplies of suitable materials can usually be obtained from packaging and cardboard manufacturers, who will cut materials to suitable sizes, or from hardware or building suppliers. The press frames should be the same size as or a little larger thatn the drying paprs. Amateur collectors often press small numbers of specimens by placing books or other weights on the pile of specimens, but this is not recommended as specimens quickly go mouldy without air circulation. The papers should be checked for dampness and changed when necessary. As the number of changes required will vary with the original succulence/water content of the plants and with the weather conditions, no exact guide can be given. Most plants should dry in less than ten days. Foir the first few days the paper should be changed daily, but after that time the frequency of changes needed depends on conditions and relative humidity levels. In tropical and wet conditions daily changes will be necessary throughout the drying period, but in drier conditions the last one or two changes need only be given at intervals of three or four days. Used paper should be discarded, or thoroughly dried again before re-use. When in the field for an extended time, drying can be aided by placing the pressed plants in a warm, sunny position during the day. In reasonably dry climates, drying is aided by securing the presses to the roof rack of the vehicle whilst driving in dry daytime conditions. If available, a hot-air fan directing air around the press will assist drying. Drying cabinets with a forced circulation of warm air are used in large herbaria to shorten drying time and to lessen the need to change drying papers, but are not necessary for small quantities of specimens. A few species regularly turn black on drying, but in general, brownish or blackish colours in the completed specimens, or the growth of mould, indicate that drying was too slow, often because the papers were not changed frequently enough in the early stages of drying. Microwave ovens Small numbers of specimens can be dried using a microwave oven. The technique recommended in the literature is to place the specimens between unprinted absorbent paper, for example butcher's paper, not newspaper, which is unsuitable because the chemicals present in the ink may cause a fire. The specimens should be put in a special press which should be of a microwave-safe material (wood, acrylic or polycarbonate sheeting e.g. plexiglass or perspex, NO metal components). If such a press is not available, sheets of cardboard can be placed above and below the specimens and then weighted down. Drying time depends on the power of your oven. In most cases drying is accomplished by irradiating at maximum power for 1-2 minutes per specimen, although it is often a case of trial and error. It is best to process no more than 10-12 specimens of average thickness per batch. Specimens are usually dried after the moisture that characteristically appears on the glass door has disappeared. If the specimen is damp when taken out of the oven, allow it to stand before re-radiating as moisture continues to evaporate from the specimen for some time. Care must be taken not to irradiate the specimens for too long. It should be noted that microwave treatment damages seeds and the cellular structure of the plants which may reduce the long-term value of the specimens. Alternative drying techniques Silica gel/other desiccants & freeze drying Alternative methods of drying plant specimens have been used for some time, but are mostly restricted to special purpose collections. The main alternatives are freeze-drying and drying in a desiccant powder such as desiccant silica gel. In general these techniques are used where it is essential to preserve the shape of a delicate plant of organ of the plant such as the flower. Freeze-drying has also been used to preserve the chemical composition of a plant as accurately as possible for later study. Disadvantages and special conservation problems of specimens dried in these manners are that they are particularly susceptible to damage. The dried parts are fragile, lack support and often catch on packing materials. They must, therefore, be packed especially carefully and stored in small boxes or tubes with some appropriate packing material that does not snag and break small projections. Acid-free tissue paper is often used. Drying in desiccant silica gel crystals or powder can also have the disadvantage that it is difficult to remove all traces of the silica gel after drying. Special preservation and processing techniques Wet or spirit collections Very fleshy or delicate structures, including small algae and orchid flowers, are best preserved in an air-tight glass or plastic jar with a liquid preservative rather than by drying. The type of preservative used should be clearly labelled in the jar. Such material is often referred to as a spirit collection or wet collection. Most material can be satisfactorily preserved in 70% ethyl alcohol (or 70% methylated spirit or denatured alcohol) with 30% water. Your pharmacist can make this up for you and it will keep indefinitely in a tightly stoppered bottle. Colours will fade quickly in spirit, however, so it is a good idea to keep comprehensive notes and photographs. Small algae Microscopic algae are often collected in a jar and in the water in which they were found. If the algae are to be stored for more than 2-3 days, a preservative needs to be used. Traditionally this has been the extremely toxic formalin - a small amount can be added to the water to make a 5% final solution, and the container labelled. This must not be sent through the post or by courier. Thee are some other equally toxic options, for example propylene phenoxytol, but none should be sent through the post. A safer option is to add sufficient concentrated alcohol or methylated spirits  to the water containing the algae to make a final solution of 70% alcohol. This treatment dilutes the algae making them difficult to find, so if they can be concentrated somehow first (e.g. by filtering) they can be stored in much less liquid. Another option is to fix the algae in formalin (or something similar) first, and then prepare a microscope glass slide with a permanent water-soluble mounting medium. See also Collecting & examining freshwater algae Some plants and certain climatic conditions require the use of specialised processing treatments. This is a brief summary: Succulent plants Very succulent plants e.g. cacti, many species of Ficus ('figs') and mistletoes drop their leaves entirely upon drying or remain alive for an excessively long period in the press. This is overcome by killing the plant before pressing, either by freezing the specimen for a few hours, dipping it in boiling water for a few minutes, or by using a microwave oven. The correct time in a microwave oven depends on the type of oven and the specimen itself, but is usually about 2 minutees. Succulent material is 'done' when it has a flaccid, water-soaked appearance. When the cell tissue has been killed (by freezing, scalding or radiation) the specimen will still require special attention until it has dried completely. The papers must be changed at least daily for the first few days, and complete drying in the case of cacti may take more than a month. An alternative technique is to place collected succulent material in 70% alcohol, as this preserves its original shape. Bulky specimens Very bulky objects (e.g. Banksia spikes, thistle heads) may be cut or sawn lengthwise before pressing. Orchids Orchids require particular care when pressing due to their delicate flowers. The flowers (at least one) should be spread out evenly so that the flower parts face the paper surface without creases or folds (never allow the parts to fold up or stick together). Alternatively, cut off each organ of a flower (three sepals, two lateral petals, a lip petal and the column attached to ovary) and spread these parts on the same piece of paper and then press. A superior method is to preserve the specimen as a spirit collection. Water plants These should be carefully laid on a sheet of paper, excess water removed, then pressed and dried in the normal way. Very soft water plants may require special treatment such as being floated onto a sheet of paper immersed in water and then dried (as is usual for marine algae) or preerving in alcohol or formalin solutions). See also Collecting & examining freshwater algae Large algae These can be kept damp for a day or so, but it is preferable to dry specimens immediately. If very soft or filamentous, such plants may be best arranged on the mounting sheet while in a dish of shallow water. The mounting sheet is placed first into the dish and specimens on the sheet then gently slid from the water. Because such specimens tend to adhere to the drying papers they are best pressed between a mounting sheet (to which the underside of the specimen may remain permanently attached) and a sheet of adhesion-resistant material (e.g. muslin) to prevent the top of the specimen adhering to the drying papers. Tropical conditions Under humid, tropical and coastal conditions special methods must be adopted to prevent rapid mould growth before the specimens can be placed in drying cabinets. Placing the entire bundle of drying papers and specimens in a plastic bag and adding a small quantity of ethyl alcohol (enough to saturate with vapour) is a method commonly adopted. This sometimes called the Schweinfurth method, after an Austrian botanist who collected extensively in tropical areas. Such methods alter specimen colours and should be avoided unless conditions make them essential. Mounting Mounting specimens prevents most fragile material from fragmenting and prevents specimens becoming separated from their labels. If the plant collection is a long-term project, specimens should be mounted on sheets of archival (permanent) cardboard or paper with archival-quality fixing media. These include stitching with cotton thread, dental floss, nickel-plated copper wire (for heavier specimens), narrow strips of archival paper, linen tape, or by using an archival adhesive such as methyl cellulose adhesive. A range of archival material is available from S& M Supply Company Pty Ltd. Dental floss can be used for bulky specimens by puncturing the sheet on either side of the specimen, threading the floss through and tying ends together in a simple reef knot. Another alternative is a clear, long-lasting 3M tape (Y8440) which is available as a special order from 3M ('Scotch brand') and their distributors. This tape has been in use in some Australian herbaria for approximately 15 years with good success. The use of tape is faster than most adhesives, and is easier to remove (by cutting and peeling from the specimen) if the specimen needs to be examined more thoroughly. Ordinary sticky tapes are unsuitable as the adhesive breaks down, becoming tacky and detached after a few years. One disadvantage of mounting specimens is that it can make parts of the specimen inaccessible for examination, so it is essential that this be borne in mind during specimen arrangement and mounting. For example, easily reversible mounting media should be used, specimens should be strapped to the sheet, rather than glued all over, and the specimen should be carefully arranged before it is attached so that it shows all features. Full-size herbarium mounting sheets are usually about 43 cm long x 28 cm wide. The plant name and accompanying field notes should be transcribed on a permanent label stuck to one corner of the herbarium sheet (the bottom right-hand corner being the most common) or, sometimes, annotations may be written directly on the sheet or card. Example specimen sheets from the NSW National Herbarium are illustrated in the diagrams. Cards 20 cm x 13 cm are a suitable size for personal reference sets of identified specimens but are unsuitable for research collections, Note: mounted specimens should not be placed in microwave ovens - adhesives often melt, and tape may ignite. Small pieces of material which may have become separated from the specimen (e.g. seeds) can be placed in small plastic bags and pinned to the sheet. Long-term preservation and storage The long-term preservation of dry plant specimens is largely dependent on protection from insect attack. Specimens collected by Linnaeus in the eighteenth century, and by Banks and Solander on the Endeavour voyage in 1788, are still excellently preserved. Pests and their control A range of pests attack dried plant material. The most common pests are insects and fungi, though rodents and other large animals can cause damage in poor storage conditions. Insects eat the material, the paper surrounding the material, and the adhesives and mounting media. Such insect pests range from psocids (book lice), which attack mainly the softer parts such as flowers and soft fruits, to tobacco beetles and carpet beetles, which can bore holes through the toughest of specimens. Many insects are particularly sensitive to relative humidity levels and do not thrive at levels below 50%. The most common and acceptable specimen treatments for insect control are: Freezing Freezing the specimens is the technique least dangerous to human health, and is very simple. The specimens must be frozen to -18oC or colder and kept at that temperature for at least 48 hours. In practice, when specimens are frozen in domestic deep-freezers in bulk and/or in boxes, it is necessary to freeze them for 72 hours (3 days and 3 nights) to ensure that the centres of thick specimens and specimens in the middle of large bundles are reduced to a low enough temperature for long enough time to kill all pests. Bundles of specimens should be sealed in plastic bags to avoid moisture condensing on the sheets as they thaw, or alternatively, dry air should be circulated around the parcel in a desiccating cabinet during re-warming. Microwave Specimens may also be treated in a microwave oven to kill any animal life present on them. Microwave treatment is a fast method for small numbers of specimens. The technique is similar to microwave drying of specimens except that a press is not essential for already dry material, and times may be reduced from those required for drying. No absolute guidelines can be given as it is best to use trial and error testing for each set of circumstances and different types of microwave, but times of 1-2 minutes per dried plant specimen should be adequate. Poisoning A traditional method of insect control was to poison the specimens with a chemical to make them unpalatable or deadly to pests. However, this is not recommended due to obvious health hazards. Domestic spray-type insecticide is of limited effectiveness and, to avoid staining, should not be sprayed directly on mounted sheets. Sprays may kill surface insects but, for instance, would not penetrate to insects living near the centre of a Banksiainfructescence or 'cone'. Many spray insecticides are now regarded as possibly detrimental to human health, so health and safety should be carefully considered before these are used. It is essential that specimens that have been poisoned be so identified, both to warn users of the health risks involved and to avoid misleading any later chemical research using the specimens. Insect deterrents A number of chemicals have been used or proposed for use as insect deterrents. Of these naphthalene (commonly found as 'moth balls') is probably the most commonly used in herbaria because of its reputation for reasonable effectiveness in insect control, coupled with low toxicity to humans. It should be noted, however, that naphthalene is poisonous if ingested, naphthalene dust can cause eye health problems for people with contact lenses, and chronic exposure is believed to be implicated in the formation of cataracts. Thee are also reports of naphtha vapour causing allergies and headaches and of possible carcinogenic effects at very high concentrations. Naphthalene in commercial quantities is most commonly available in flake or chip form. If left loose in containers/boxes it is more readily inhaled or ingested and is more likely to case problems to people with contact lenses than is naphthalene in block or ball form or naphtha flakes or chips encased in porous bags or boxes. If naphthalene is used as an insect deterrent the levels around specimens must be maintained at a steady, level to ensure effective insect control. Because of the exposure limits for humans this is best done by storing specimens in boxes or in a sealed cupboard. Fungal pests Fungal (mould) attack is mainly a danger to damp specimens, either through incomplete drying during specimen preparation, or to collections that become wet later through flood, other water damage or improper storage conditions. Properly dried plant specimens will not suffer from fungal attack if stored in the correct conditions (see recommendations below) though freeze-dried fungal bodies such as mushrooms have been reported to be very susceptible to mould growth. Specimens with sugary exudations or large quantities of nectar are also particularly attractive to fungi, and need special care during drying to ensure that they dry fast enough to prevent mould growth. If fungus grows on the specimens these can be brushed with alcohol or methylated spirits (denatured alcohol). However, this may alter the specimen unacceptably for chemical and other investigative research, and only kills the fungus present on the specimen; it does not correct the problems that allowed the fungus to develop. Specimens treated for fungal attack should be clearly annotated as such, including date and treatment given. Storage Dried and pressed plant specimens can be stored in cardboard or plastic boxes, or tied in bundles in light-weight cardboard folders placed in 'pigeon holes'. Alternatively, they can be placed in protective plastic jackets and displayed in ring folders which is recommended if they are to be frequently handled, such as for a reference collection. Filing Specimens should be filed in a systematic order if a relatively permanent collection is being made. The major groups, i.e. ferns and fern allies, cycads, conifers, dicotyledons and monocotyledons, are best kept separately or according to some classification scheme, such as that given in a flora or handbook. Similarly, the genera within each family and the species within each genus may be filed alphabetically or following some such classification.